Figure 2.

All analyzed mDA neuron markers are lost or diminished in cNurr1^DATCre mice. A–T, Confocal microscopy showing immunohistochemistry of several different mDA neuron markers in control (ctrl) and in cNurr1^DATCre mice at E15.5 or at P1, as indicated. The following markers were analyzed: DAT, AADC, VMAT, Pitx3, and Lmx1b. Results show a progressive loss of markers that is more substantial in the lateral SNc, whereas more medial VTA cells are lost more slowly. DAT is completely absent already at E15.5 in cNurr1^DATCre mice, whereas all other markers are decreased more slowly in this area (compare A, B). Scale bars, 200 μm.