Skip to main content
. 2009 Feb 25;29(8):2597–2610. doi: 10.1523/JNEUROSCI.5439-08.2009

Figure 5.

Figure 5.

Actograms of so1 and somda mutants and TIM staining in the clock neurons of both mutants at two times on days 3–4 in DD (red and blue points). Ten flies were stained at each time for each mutant, respectively. As can be seen in the example actograms, so1 mutants show one rhythm free-running with long period as soon as released to DD (A), whereas somda mutants reveal two rhythmic components in DD, which free-run with short and long periods, respectively, and that were 180° out of phase at the times of staining (C). B, In so1 mutants, the CRY-positive clock neurons were strongly TIM-immunoreactive in the early subjective morning, just before the flies became active (A, red point; B, red columns) and less stained in the subjective evening, when the flies were most active (A, blue point; B, blue columns). The CRY-negative neurons did not show this difference. ANOVA revealed a significant influence of the time of staining on labeling intensity (F (1,165) = 30.68; p < 0.001) and that this influence was dissimilar in the different neuronal groups (F (7,165) = 3.96; p < 0.001). The post hoc test showed that only the CRY-positive neurons showed significantly higher TIM staining in the early subjective morning compared with the evening. No significant differences were revealed for the CRY-negative neurons. D, In somda mutants, ANOVA showed again a significant influence of time on staining intensity (F (1,179) = 4.62; p = 0.03), which was strongly dissimilar in the different neurons (F (8,179) = 34.78; p < 0.001). No significant staining differences were only found in the CRY-negative DN1p remain. The other neurons were either strongly TIM-immunoreactive at the activity maximum of the long-period component (C, blue point; D, blue columns) or at the activity maximum of the short-period component (C, red point; D, red columns). For additional explanations, see text. The green point in C indicates the time at which short- and long-period components crossed each other and were consequently in phase again. At this day, the different clock neurons cycled in synchrony with each other (supplemental Fig. S1, available at www.jneurosci.org as supplemental material). Error bars indicate SEM.