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TASK3 gene inactivation. A, Inactivation strategy. Open triangles represent loxP sequences. Exon 2 is deleted in KO mice. B, PCR analysis of genomic DNA. Primer positions are shown in A. C, TASK3 immunodetection in adult mouse cerebellum. TASK3 expression observed in the molecular layer (Mol) of wild-type (WT) mouse was lacking in KO mouse. Gr, Granule cell layer; Pk, Purkinje cells.
