Figure 4.

TREK2 gene inactivation. A, Inactivation strategy. Exon 2 is duplicated in KO mice introducing a frameshift resulting in a premature stop codon. B, PCR analysis of genomic DNA from mutant mice. Primer positions are shown in A. C, RT-PCR analysis of brain cDNAs. Primer positions are shown in A. Multiple PCR products are amplified from KO animals that correspond to alternative hybridizations from the exon 2 repeat. D, Western analysis of adult mouse brain proteins using anti-TREK2 antibodies.