Skip to main content
. 2009 Feb 11;29(6):1937–1946. doi: 10.1523/JNEUROSCI.5343-08.2009

Figure 5.

Figure 5.

p11 facilitates signaling through 5-HTR4. a, Effect of 5-HT (1 μm) on cAMP levels in COS7 cells transfected with p11, 5-HTR4, p11 and 5-HTR4, 5-HTR6, or p11 and 5-HTR6. b, Effect of stimulation with 5-HTR4 partial agonist RS67333 (10 μm) in primary neuronal cultures derived from p11 WT or KO mice. Increase in cAMP levels in response to RS67333 was blunted by >50% in p11 KO cultures compared with WT. c, Quantification and representative image from Western blotting analysis of transfected COS7 cells in the presence of serotonin (1 μm). Cotransfection of p11 with 5-HTR4 increased phosphorylation of MAPK compared with 5-HTR4 alone (1 = mock, 2 = p11, 3 = 5-HTR4, 4 = 5-HTR4 and p11, 5 = 5-HTR6, and 6 = 5-HTR6 and p11). d, Primary cortical neuronal cultures derived from p11 WT or KO mice stimulated with RS67333. RS67333 increased phosphorylation of MAPK in WT, but not in KO cultures. All data are presented as mean ± SEM, *p < 0.05, **p < 0.01.