Figure 3.

HVEM mediates the inhibitory effect of LIGHT on BDNF-promoted neurite growth from nodose neurons. A, B, Dissociated cultures of P0 nodose neurons were transfected 3 h after plating with a YFP expression plasmid together with a plasmid expressing either LIGHT, LIGHT-R228E, or LIGHT-G119E or with an empty control plasmid (control). After a further 24 h incubation in medium containing BDNF (10 ng/ml), confocal images of neurite arbors were acquired for calculation of total neurite length (A) and Sholl analysis (B). C, D, Dissociated cultures of P0 nodose neurons were transfected 3 h after plating with a YFP expression plasmid together with either a LIGHT expression plasmid or an empty control plasmid. After a further 24 h incubation in medium containing BDNF (10 ng/ml) with and without anti-HVEM blocking antibody (1:50), confocal images of neurite arbors were acquired for calculation of total neurite length (C) and Sholl analysis (D). The combined data from three separate experiments of each kind are plotted (means and SEs of the data from >150 neurons for each data point). Statistically significant difference with control-transfected neurons: ***p < 0.001 (A). Statistically significant difference with LIGHT-transfected neurons: ****p < 0.0001 (C).