Figure 4.

A–D, Endogenous HVEM signaling exerts a neurite growth inhibitory effect on nodose neurons. Dissociated cultures of P0 nodose neurons were incubated with and without anti-HVEM blocking antibody (1:50) in medium containing the caspase inhibitor Boc-d-FMK (25 μm) and either no BDNF, 0.1 ng/ml BDNF, or 1 ng/ml BDNF. After 24 h incubation the neurons were labeled with calcein-AM and confocal images of neurite arbors were acquired for calculation of total neurite length (A) and Sholl analysis (B–D). The combined data from three separate experiments of each kind are plotted (means and SEs of the data from >150 neurons for each data point). Statistically significant differences in neurite length in cultures with and without anti-HVEM: **p < 0.01, ***p < 0.001.