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. Author manuscript; available in PMC: 2020 Jun 11.
Published in final edited form as: Biochemistry. 2019 May 24;58(23):2670–2674. doi: 10.1021/acs.biochem.9b00275

Figure 2.

Figure 2.

Multi-granulin domain peptides reduce the melting temperature of proCTSD. Derivatives of the raw DSF fluorescent intensity versus temperature are plotted for 1 μM proCTSD in the absence and presence of: (A) 2 μM PGRN, (B) 2 μM peptide pG, (C) 2 μM peptide BAC, (D) 2 μM peptide CDE, and (E) granulin C. DSF was performed at neutral pH 7.4 with 1× Sypro Orange. Assays were run in triplicate and data plotted are mean + SEM.