. Author manuscript; available in PMC: 2020 Jun 11.

Published in final edited form as: Biochemistry. 2019 May 24;58(23):2670–2674. doi: 10.1021/acs.biochem.9b00275

Table 1.

Summary table showing binding and activity of multi-granulin domain peptides on proCTSD.^a

	Neutral pH (7.4)		Acidic pH (3.5)
Peptide	MST - K_d (μM)	DSF - ΔT_m (°C)	Normalized V_max	Lag time (sec)
proCTSD	-	-	1.00 ± 0.00	1712 ± 31.42
+ PGRN	0.95 ± 0.34	−0.41 ± 0.05	1.29 ± 0.06	1585 ± 34.65
+ pG	0.33 ± 0.03	−0.23 ± 0.02	1.27 ± 0.11	1611 ± 30.01
+ BAC	2.80 ± 0.89	−3.38 ± 0.08	1.89 ± 0.09^***	1343 ± 51.57^**
+ CDE	0.13 ± 0.01	−2.78 ± 0.36	1.84 ± 0.09^***	1376 ± 48.23^*
+ C	-	0.05 ± 0.07	1.13 ± 0.17	1488 ± 117.20

Values shown are mean ± SEM. Normalized V_max and lag time were analyzed for significant difference to proCTSD alone using one-way ANOVA with Tukey’s multiple comparisons test,

^***

= P<0.001,

^**

= P<0.01,

= P<0.05.

The +PGRN condition is significantly different for V_max when compared with a Student’s t-test (P = 0.0076).