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. Author manuscript; available in PMC: 2019 Jul 30.
Published in final edited form as: J Phys Chem B. 2016 Jun 15;120(26):6430–6438. doi: 10.1021/acs.jpcb.6b04525

Figure 2: shows how the complex ESIMS spectrogram of the Au102 fraction can be simplified into a single peak using Protein Deconvolution 4.0 software.

Figure 2:

The raw negative ion complex spectra of a 50:50 methanol/water solution of the (102,44) fraction was analyzed using Manual Xtract (isotopically resolved) at 4 ppm mass tolerance, 99% confidence, 2–100kDa outpout mass range, and intact protein peak model mode. Four charge states where detected (z = 6- to 9-). The mass tolerance was relaxed to 20ppm to detect the sodium adducts depicted in the inset. Infusion rate = 5 μL/min; particle concentration ~ 0.5 μg/μL; scan duration = 1.0 minute.