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. 2019 Jul 26;11:7077–7087. doi: 10.2147/CMAR.S209940

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© 2019 Kondratyeva et al.

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Construction of PDX1 overexpression in PANC-1 cells. (A) Scheme of the lentiviral construct used to obtain GFP-labeled PANC-1^PDX1 (1) and PANC-1^Control (2) cells. (B) Western blot analysis of the expression of the PDX1 protein in PANC-1^PDX1 cells and in the PANC-1^Control group, and the expression of PDX1 in PANC-1^PDX1 after treatment with anti-PDX1 siRNA and negative siRNA. Abbreviations: PCNA - proliferating cell nuclear antigen gene promoter; PDX1 -Pancreatic And Duodenal Homeobox 1 gene; PGK - promoter of 3-phosphoglycerate kinase gene; puro - puromycin-resistance gene encoding N-acetyl-transferase; CMV - cytomegalovirus promoter; GFP - green fluorescent protein gene, 5'-LTR, 3'- LTR - 5' and 3' long terminal repeat; GAPDH - Glyceraldehyde 3-phosphate dehydrogenase; kDa - kilodalton.