Table 1.

Real-time PCR primers used in gene expression analysis

Gene	Primer sequence (5’ → 3’)		PCR product (bp)
18S	Fw	CGCGGTTCTATTTTGTTGGT	501
Rv	ATGCCAGAGTCTCGTTCGTT
EEF1a	Fw	GACACGTAGATTCGGGCAAG	173
Rv	GATACCACGTTCACGCTCA
PDX1	Fw	GTCCTGGAGGAGCCCAAC	272
Rv	CGGCGGTTTTGGAACCAGAT
CDH1	Fw	AGTGCCTGCTTTTGATGATG	338
Rv	AGCTTGAACTGCCGAAAAATC
KRT8	Fw	ATGTTGTCCATGTTGCTTCG	125
Rv	ACCCTCAACAAGTTTGCC
MUC1	Fw	CTGGTCTGTGTTCTGGTTGC	250
Rv	CCACTGCTGGGTTTGTGTAAG
KLF5	Fw	ACAAATCAGACAGCAGCAATGGACA	312
Rv	GGTGGTGGGTAAATTTGGATTGTGA
VIM	Fw	GCAGAAGAATGGTACAAATCCA	144
Rv	TTTAAGGGCATCCACTTCACA
SNAIL	Fw	CCAATCGGAAGCCTAACTAC	124
Rv	GCGGTGGGGTTGAGGATCTC
SLUG	Fw	AGAAGGTTTTGGAGCAGTTTTTG	160
Rv	TGGTTGCTTCAAGGACACAT
ZEB1	Fw	GAACAGTGTTCCATGCTTAAGAGCG	217
Rv	GGGCGGTGTAGAATCAGAGTCATTC

Abbreviations: Fw, forward primer; Rw, reverse primer; bp, base pair.