Table 1.
Genes | Primers (forward/reverse) |
---|---|
HOXA10 | 5ʹ- AGAGATTAGCCGCAGCGTCC −3ʹ and 5ʹ- TTCCTGGGCAGAGCCTGAAG −3’ |
HDAC1 | 5ʹ-CTACTACGACGGGGATGTTGG-3ʹ and 5ʹ- GAGTCATGCGGATTCGGTGAG −3ʹ |
HDAC2 | 5ʹ- ATGGCGTACAGTCAAGGAGG −3ʹ and 5ʹ- TGCGGATTCTATGAGGCTTCA −3ʹ |
HDAC3 | 5ʹ- TCTGGCTTCTGCTATGTCAACG-3ʹ and 5ʹ- CCCGGTCAGTGAGGTAGAAAG −3ʹ |
GAPDH | 5ʹ- CACCCACTCCTCCACCTTTG −3ʹ and 5ʹ-CCACCACCCTGTTGCTGTAG-3ʹ |
HDAC1 ChIP-primer 1 | 5ʹ-AAAGAAAGGAAACCTGCCCTC-3ʹ and 5ʹ-TGCAGTCACCCAGGATGACTA-3ʹ |
HDAC1 ChIP-primer 2 | 5ʹ- AGTGCCTCTGGTGCAAAGAA −3ʹ and 5ʹ- TCGGCCAAGGGCTTTTTA −3ʹ |
HDAC1 ChIP-primer 3 | 5ʹ-CCGCGTGTTGCTTTTCTTTT-3ʹ and 5ʹ- TGAAAAGCGTGCTTGGAGAT −3ʹ |
Abbreviations: qRT-PCR, quantitative real-time PCR; HOXA10, homeobox A10; HDAC, histone deacetylase; GAPDH, glyceraldehyde 3-phosphate dehydrogenase; ChIP, chromatin immunoprecipitation.