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. Author manuscript; available in PMC: 2019 Jul 30.
Published in final edited form as: Cold Spring Harb Protoc. 2011 May 1;2011(5):pdb.prot5611. doi: 10.1101/pdb.prot5611

FIGURE 2.

FIGURE 2.

Effects of incubation times on protein recovery and nonspecific interactions. Coomassie-stained SDS-PAGE showing isolation of GFP-tagged Nup84 and its associated proteins using various incubation times (left). Recoveries of the indicated proteins as a function of time. Proteins were identified by mass spectrometry (right). (Reprinted, with permission, from the American Society for Biochemistry and Molecular Biology, Inc.)