Skip to main content
. Author manuscript; available in PMC: 2019 Jul 30.
Published in final edited form as: Cell Rep. 2019 Jul 23;28(4):877–895.e6. doi: 10.1016/j.celrep.2019.06.074

Figure 2. Epitope-Specific mAbs Define the Antigenicity of V1V2 and V3 Scaffolds.

Figure 2.

V1V2-specific mAbs were serially diluted and assayed by ELISA in duplicate at a starting concentration of 5 μg/mL to evaluate the antigenicity of each immunogen. Curves are mean ± SEM.

(A-F). Each mAb and specificity is shown: PG9 V2q (A), 830A V2i (B), 2158 V2i (C), 697D V2i (D), CH58 V2p (E), CH59 V2p (F). Unique symbols identify each V1V2-scaffold protein.

(G) Binding to the V3(ZM109)-TTB scaffold with V3-specific mAbs 447–52D and 2219 and control V2 and CD4bs mAbs.

(H) EC50 values derived from ELISA curves in (A)–(G).

Data are representative of assays repeated at least twice with comparable results.