Figure 3.

Inhibition of polyamine synthesis in vivo attenuates both the ongoing polyamine synthesis-dependent and -independent growth triggered by a peripheral lesion. At the same time as the sciatic nerve was lesioned, intrathecal minipumps were inserted that delivered 0.5 μmol/h DFMO for the entire time until the animals were killed, and the DRGs were removed and cultured on either MAG-expressing (striped bars) or control CHO (black bars) cells. Saline was delivered intrathecally as a control, and results were compared with neurite outgrowth of DRG neurons from quantitation of neurite outgrowth. Results represent the length of the longest neurite from ∼200 neurons ± SEM, as percentage of neurite length of neurons from unlesioned, contralateral controls grown on control CHO cells. C, Control.