Figure 7.

Intrathecal delivery of spermidine overcomes inhibition by MAG and myelin when neurons are cultured. Spermidine was delivered via intrathecal minipumps at a rate of 25 nmol/h for 4 d before the DRG neurons were removed and cultured overnight on either MAG-expressing CHO cells (striped bars) or control CHO cells (black bars), after which they were fixed and stained for β-III tubulin. Quantitation of neurite length for the longest neurite from ∼200 neurons ± SEM. ***p ≥ 0.001 compared with neurite length on MAG cells.