Figure 2.

Sensitivity of memory formation to protein synthesis inhibition after extinction with one CS-only trial does not depend on the US duration during training. On day 1, animals were subjected to three CS–US pairings (A1–A3) with a short (2 s, circles) or long (10 s, triangles) US presentation (acquisition). After 24 h, on day 2, emetine (black symbols) or PBS (gray symbols) was injected 30 min before one CS-only trial (E1). On day 3, memory was tested (retention). Presented are percentages of bees showing the CR to the CS at different phases of the experiment. Arrow, Time of injection. Number of animals: long-Eme, n = 94; long-PBS, n = 98; short-Eme, n = 74; short-PBS, n = 90.