Table 2.
Analysis of the predicted ORFs of vB_SflS-ISF001 and their putative functions.
| Best match (NCBI database) | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| ORFs | Strand | Left | Right | Start codon | Size (aa) | PI | Mw (Kda) | Predicted product [organism] | E value | Identity | Accession no |
| 1 | + | 209 | 616 | ATG | 135 | 9.2 | 16166.87 | Hypothetical protein T1p10 [Escherichia virus T1] | 7E-90 | 95% | YP_003935.1 |
| 2 | + | 806 | 1021 | ATG | 71 | 4.78 | 8176.19 | Hypothetical protein B508_00390 [Escherichia phage ADB-2] | 3E-35 | 83% | YP_007112743.1 |
| 3 | - | 1035 | 1436 | ATG | 133 | 8.89 | 13972.21 | Hypothetical protein JMPW1_065 [Escherichia phage JMPW1] | 1E-73 | 89% | ALT58269.1 |
| 4 | - | 1436 | 1924 | ATG | 162 | 9.35 | 18135.87 | Endolysin [Shigella phage SH6] | 2E-101 | 90% | APC44908.1 |
| 5 | - | 1924 | 2139 | ATG | 71 | 6.06 | 7645.94 | Putative holin [Escherichia virus T1] | 6E-36 | 90% | YP_003932.1 |
| 6 | - | 2498 | 3649 | ATG | 383 | 6.54 | 43031.97 | Hypothetical protein JMPW1_061 [Escherichia phage JMPW1] | 0 | 92% | ALT58265.1 |
| 7 | - | 3728 | 4015 | ATG | 95 | 7.84 | 10788.54 | Hypothetical protein B508_00365 [Escherichia phage ADB-2] | 4E-55 | 88% | YP_007112738.1 |
| 8 | - | 4210 | 4434 | ATG | 74 | 9.73 | 8432.87 | Hypothetical protein ISF001_007 [Shigella phage vB_SsoS-ISF002] | 9E-39 | 88% | ASD50891.1 |
| 9 | - | 4486 | 4734 | ATG | 82 | 4.67 | 9713.19 | Hypothetical protein ISF001_008 [Shigella phage vB_SsoS-ISF002] | 8E-45 | 90% | ASD50892.1 |
| 10 | - | 4742 | 5455 | ATG | 273 | 6.84 | 26966.43 | DNA adenine methyltransferase [Escherichia phage vB_EcoS_SH2] | 3E-147 | 87% | ARW57245.1 |
| 11 | - | 5523 | 5939 | ATG | 138 | 8.59 | 15797.96 | Hypothetical protein ISF001_0010 [Shigella phage vB_SsoS-ISF002] | 4E-98 | 100% | ASD50894.1 |
| 12 | - | 5936 | 7948 | ATG | 670 | 6.59 | 75636.28 | ATP-dependent helicase [Escherichia phage JMPW2] | 0 | 95% | ALT58178.2 |
| 13 | + | 8048 | 8500 | ATG | 150 | 10.47 | 16913.61 | Hypothetical protein Shfl1p58 [Shigella virus Shfl1] | 9E-93 | 90% | YP_004414874.1 |
| 14 | + | 8577 | 9497 | ATG | 306 | 6.04 | 34833.12 | DNA primase/helicase [Escherichia phage JMPW1] | 0 | 91% | ALT58257.1 |
| 15 | + | 9598 | 11382 | ATG | 594 | 4.8 | 64226.31 | Putative tail fiber [Shigella virus Shfl1] | 0 | 87% | YP_004414872.1 |
| 16 | - | 11411 | 11824 | ATG | 137 | 7.87 | 15667.35 | Single-stranded DNA-binding protein [Shigella phage SH6] | 1E-62 | 78% | APC44921.1 |
| 17 | - | 11870 | 12517 | ATG | 215 | 8.52 | 23707.2 | Putative recombination protein [Shigella phage vB_SsoS-ISF002] | 8E-128 | 91% | ASD50900.1 |
| 18 | - | 12592 | 13656 | ATG | 354 | 5.02 | 39954.22 | Exodeoxyribonuclease VIII [Shigella phage SH6] | 0 | 93% | APC44928.1 |
| 19 | + | 14184 | 14414 | ATG | 76 | 8.71 | 8399.77 | Phage lipoprotein [Shigella phage SH6] | 6E-38 | 84% | APC44941.1 |
| 20 | + | 14417 | 15373 | ATG | 318 | 8.09 | 34098.65 | Hypothetical protein pSf2_021 [Shigella phage pSf-2] | 0 | 94% | YP_009112959.1 |
| 21 | - | 15468 | 18851 | ATG | 1127 | 4.88 | 125022.94 | Tail fiber protein [Shigella phage SH6] | 0 | 94% | APC44985.1 |
| 22 | - | 18929 | 19528 | ATG | 199 | 9.1 | 20875.01 | Putative tail assembly protein [Escherichia phage ADB-2] | 9E-135 | 96% | YP_007112720.1 |
| 23 | - | 19525 | 20259 | ATG | 244 | 5.74 | 28258.09 | Putative minor tail protein [Escherichia virus T1] | 0 | 99% | YP_003910.1 |
| 24 | - | 20256 | 21038 | ATG | 260 | 8.52 | 28774.74 | Putative minor tail protein [Escherichia virus T1] | 2E-173 | 90% | YP_003909.1 |
| 25 | - | 21118 | 21471 | ATG | 117 | 4.64 | 13011.49 | Tail fiber protein [Escherichia phage JMPW1] | 6E-72 | 87% | ALT58245.1 |
| 26 | - | 21474 | 24347 | ATG | 957 | 6.05 | 103770.01 | Tail length tape measure protein [Escherichia phage JMPW2] | 0 | 94% | ALT58162.1 |
| 27 | - | 24387 | 24656 | ATG | 89 | 4.25 | 10131.71 | Hypothetical protein pSf2_028 [Shigella phage pSf-2] | 7E-49 | 89% | YP_009112966.1 |
| 28 | - | 24704 | 25021 | ATG | 105 | 6.72 | 11815.39 | Hypothetical protein pSf2_029 [ Shigella phage pSf-2] | 4E-53 | 88% | YP_009112967.1 |
| 29 | - | 25136 | 25804 | ATG | 222 | 5.07 | 24090.32 | Putative major tail protein [Shigella virus Shfl1] | 2E-140 | 88% | YP_004414858.1 |
| 30 | - | 25807 | 26205 | ATG | 132 | 9.07 | 15330.60 | Hypothetical protein pSf2_031 [Shigella phage pSf-2] | 3E-79 | 87% | YP_009112969.1 |
| 31 | - | 26195 | 26638 | ATG | 147 | 6.91 | 16523.76 | Hypothetical protein Shfl1p40 [Shigella virus Shfl1] | 9E-96 | 91% | YP_004414856.1 |
| 32 | - | 26631 | 27002 | ATG | 123 | 5.69 | 13904.7 | Hypothetical protein pSf2_033 [Shigella phage pSf-2] | 2E-76 | 92% | YP_009112971.1 |
| 33 | - | 26999 | 27412 | ATG | 137 | 9.25 | 15542.96 | Hypothetical protein JMPW2_033 [Escherichia phage JMPW2] | 8E-79 | 85% | ALT58155.2 |
| 34 | - | 27455 | 27745 | ATG | 96 | 4.76 | 10348.09 | Hypothetical protein T1p46 [Escherichia virus T1] | 2E-46 | 82% | YP_003899.1 |
| 35 | - | 27795 | 28754 | ATG | 319 | 6.61 | 35068.32 | Hypothetical protein Shfl1p36 [Shigella virus Shfl1] | 0 | 93% | YP_004414852.1 |
| 36 | - | 28847 | 29614 | ATG | 255 | 4.65 | 26691.97 | Hypothetical protein Shfl1p35 [Shigella virus Shfl1] | 1E-144 | 81% | YP_004414851.1 |
| 37 | - | 29674 | 30150 | ATG | 158 | 5.54 | 17268.48 | Hypothetical protein pSf2_038 [Shigella phage pSf-2] | 9E-89 | 83% | YP_009112976.1 |
| 38 | - | 30162 | 31274 | ATG | 370 | 5.33 | 40269.73 | Major head subunit precursor [Escherichia virus T1] | 0 | 92% | YP_003895.1 |
| 39 | - | 31277 | 32038 | ATG | 253 | 9.09 | 28826.61 | Minor capsid protein [Escherichia phage JMPW1] | 7E-163 | 90% | ALT58231.1 |
| 40 | - | 32028 | 33311 | ATG | 427 | 4.71 | 47760.74 | Putative portal protein [Shigella virus Shfl1] | 0 | 93% | YP_004414847.1 |
| 41 | - | 33368 | 34936 | ATG | 522 | 6.91 | 59967.82 | Putative terminase large subunit [Shigella virus Shfl1] | 0 | 94% | YP_004414846.1 |
| 42 | - | 34975 | 35499 | ATG | 174 | 4.93 | 19287.68 | Putative terminase small subunit [Escherichia virus T1] | 3E-114 | 93% | YP_003891.1 |
| 43 | - | 35584 | 35811 | ATG | 75 | 9.39 | 8557.22 | Hypothetical protein Shfl1p28 [Shigella virus Shfl1] | 4E-33 | 88% | YP_004414844.1 |
| 44 | - | 35813 | 35998 | ATG | 61 | 9.57 | 7039.22 | Hypothetical protein JMPW1_022 [Escherichia phage JMPW1] | 2E-25 | 80% | ALT58226.1 |
| 45 | - | 35979 | 36140 | ATG | 53 | 9.22 | 5894.76 | Hypothetical protein pSf2_046 [Shigella phage pSf-2] | 3E-20 | 83% | YP_009112984.1 |
| 46 | - | 36305 | 36508 | ATG | 67 | 5.07 | 7230.12 | Hypothetical protein B508_00150 [Escherichia phage ADB-2] | 4E-34 | 84% | YP_007112695.1 |
| 47 | - | 36508 | 36738 | ATG | 76 | 9.75 | 8737.25 | Hypothetical protein JMPW1_019 [Escherichia phage JMPW1] | 7E-39 | 88% | ALT58223.1 |
| 48 | - | 36738 | 37082 | ATG | 114 | 9.16 | 12972 | Hypothetical protein B508_00140 [Escherichia phage ADB-2] | 1E-65 | 87% | YP_007112693.1 |
| 49 | - | 37079 | 37288 | ATG | 69 | 4 | 8032.77 | Hypothetical protein B508_00135 [Escherichia phage ADB-2] | 1E-33 | 83% | YP_007112692.1 |
| 50 | - | 37361 | 37933 | ATG | 190 | 5.55 | 21575.59 | Hypothetical protein T1p62 [Escherichia virus T1] | 2E-123 | 91% | YP_003883.1 |
| 51 | - | 38042 | 38575 | ATG | 177 | 5.95 | 20038.7 | Putative morphogenetic protein [Escherichia phage ADB-2] | 6E-112 | 90% | YP_007112690.1 |
| 52 | - | 38659 | 39105 | ATG | 148 | 8.51 | 17383.97 | Hypothetical protein SH6_0017 [Shigella phage SH6] | 2E-81 | 95% | APC44930.1 |
| 53 | - | 39163 | 39381 | ATG | 72 | 4.75 | 7840.97 | Hypothetical protein T1p66 [Escherichia virus T1] | 5E-33 | 83% | YP_003878.1 |
| 54 | - | 39530 | 40168 | ATG | 212 | 9.38 | 23844.42 | Hypothetical protein pSf2_055 [Shigella phage pSf-2] | 9E-138 | 91% | YP_009112993.1 |
| 55 | - | 40173 | 40460 | ATG | 95 | 7.84 | 11139.68 | Hypothetical protein B508_00110 [Escherichia phage ADB-2] | 7E-50 | 81% | YP_007112687.1 |
| 56 | - | 40539 | 40688 | ATG | 49 | 7.82 | 5667.66 | Hypothetical protein [Escherichia phage vB_EcoS_SH2] | 5E-25 | 88% | ARW57197.1 |
| 57 | - | 40688 | 41194 | ATG | 168 | 6.96 | 18812.91 | Hypothetical protein pSf2_059 [Shigella phage pSf-2] | 3E-88 | 77% | YP_009112997.1 |
| 58 | - | 41266 | 41754 | ATG | 162 | 4.43 | 18232.68 | Hypothetical protein B508_00095 [Escherichia phage ADB-2] | 2E-98 | 89% | YP_007112684.1 |
| 59 | - | 41826 | 42017 | ATG | 63 | 4.05 | 7383.14 | Hypothetical protein JMPW2_006 [Escherichia phage JMPW2] | 3E-29 | 84% | ALT58128.1 |
| 60 | - | 42027 | 42200 | ATG | 57 | 6.52 | 6161.42 | Hypothetical protein ISF001_0059 [Shigella phage vB_SsoS-ISF002] | 5E-24 | 86% | ASD50943.1 |
| 61 | - | 42304 | 42531 | ATG | 75 | 10.07 | 8613.07 | Hypothetical protein JMPW2_004 [Escherichia phage JMPW2] | 2E-30 | 68% | ALT58126.1 |
| 62 | - | 42538 | 42768 | ATG | 76 | 6.54 | 8632.82 | Hypothetical protein Shfl1p05 [Shigella virus Shfl1] | 5E-39 | 88% | YP_004414824.1 |
| 63 | - | 42847 | 43317 | ATG | 156 | 5.51 | 17590.09 | Hypothetical protein B508_00070 [Escherichia phage ADB-2] | 4E-77 | 74% | YP_007112679.1 |
| 64 | - | 43320 | 43514 | ATG | 64 | 5.1 | 7308.31 | Hypothetical protein Shfl1p02 [Shigella virus Shfl1] | 2E-29 | 86% | YP_004414821.1 |
| 65 | - | 43586 | 43915 | ATG | 109 | 5.7 | 12370.27 | Hypothetical protein ISF001_0064 [Shigella phage vB_SsoS-ISF002] | 2E-60 | 87% | ASD50948.1 |
| 66 | - | 43928 | 44503 | ATG | 191 | 4.99 | 21342.21 | Hypothetical protein JMPW2_001 [Escherichia phage JMPW2] | 5E-104 | 81% | ALT58123.1 |
| 67 | + | 45206 | 45910 | ATG | 234 | 9.14 | 26394.97 | DNA methylase [Shigella phage SH6] | 6E-146 | 89% | APC44923.1 |
| 68 | + | 45971 | 46156 | ATG | 61 | 9.16 | 7030.22 | Hypothetical protein B508_00040 [Escherichia phage ADB-2] | 2E-24 | 79% | YP_007112675.1 |
| 69 | + | 46172 | 46357 | ATG | 61 | 6.14 | 6920.14 | Hypothetical protein ISF001_0068 [Shigella phage vB_SsoS-ISF002] | 9E-25 | 82% | ASD50952.1 |
| 70 | + | 46433 | 46813 | ATG | 126 | 4.49 | 14559.40 | Hypothetical protein ISF001_0069 [Shigella phage vB_SsoS-ISF002] | 2E-66 | 83% | ASD50953.1 |
| 71 | + | 46810 | 46983 | ATG | 57 | 8.01 | 6695.60 | Hypothetical protein ISF001_0070 [Shigella phage vB_SsoS-ISF002] | 4E-20 | 75% | ASD50954.1 |
| 72 | + | 47055 | 47426 | ATG | 123 | 4.51 | 13557.28 | Hypothetical protein JMPW1_074 [Escherichia phage JMPW1] | 4E-61 | 80% | ALT58278.1 |
| 73 | + | 47419 | 47619 | ATG | 66 | 6.18 | 7624.69 | Hypothetical protein T1p02 [Escherichia virus T1] | 2E-28 | 82% | YP_003943.1 |
| 74 | + | 47637 | 47957 | ATG | 106 | 9.71 | 12105.10 | Hypothetical protein pSf2_078 [Shigella phage pSf-2] | 9E-56 | 81% | YP_009113016.1 |
| 75 | + | 48174 | 48398 | ATG | 74 | 10.16 | 7966.25 | Hypothetical protein B508_00015 [Escherichia phage ADB-2] | 1 | 85% | YP_007112670.1 |
| 76 | + | 48402 | 48614 | ATG | 70 | 3.93 | 8105.68 | Hypothetical protein T1p06 [Escherichia virus T1] | 1E-25 | 71% | YP_003939.1 |
| 77 | + | 48695 | 49057 | ATG | 120 | 9.62 | 13975.26 | Hypothetical protein B508_00005 [Escherichia phage ADB-2] | 1E-70 | 90% | YP_007112668.1 |
| 78 | + | 49188 | 50009 | ATG | 273 | 5.89 | 30123.19 | Hypothetical protein pSf2_083 [Shigella phage pSf-2] | 0 | 98% | YP_009113021.1 |