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. 2019 Jul 30;14(7):e0220393. doi: 10.1371/journal.pone.0220393

Fig 6. Inhibition of Hsp90 decreased cellular apoptosis.

Fig 6

(A) Differentiated SH-SY5Y cells were transiently co-transfected with expression vector for GFP-68Q and 12 μM Hsp90 ASO or 12 μM Hsp90 mutant oligonucleotide. Their effects on cell viability were analyzed up to 48 h. Transfected cells with expression vector for GFP-480-17Q are normalized to 0% (P<0.05). (B) Cells lysates were analyzed by Western blotting, β-actin was used to assess total protein loading. (C) Protein levels were compared to control cells, which were only transfected with expression vector for GFP-68Q. (D) Quantitative analysis to determine the percentage of cells that presented to mHtt in nucleus. The results represent the mean and SEM (n = 100) from a series of three separate experiments (P<0.05).