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. 2019 Jul 30;14(7):e0220389. doi: 10.1371/journal.pone.0220389

Fig 1. Generation of miR-137 Tg mice and altered gene expression of the putative targets for miR-137.

Fig 1

(A) Transgene constructs. The genomic region encompassing mature miR-137 was ligated into the XhoI site of the plasmid containing the Thy-1 promoter. The Thy-1 promoter/miR-137 plasmid was linearized using restriction enzyme digestion and was microinjected into C57BL/6J fertilized eggs. (B) Expression level of miR-137 in several brain regions in WT and miR-137 Tg mice. Data are expressed as the mean + SEM (3-month-old male mice, n = 3). (C) Comparison of transcriptional changes of the miR-137 Tg mice at 3 months of age and mmu-miR-137-predicted target genes. Enrichment p-values were calculated in each brain region. mPFC, medial prefrontal cortex; Str, striatum; Thal, thalamus; Hip, hippocampus; Cb, cerebellum.