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. Author manuscript; available in PMC: 2019 Jul 30.
Published in final edited form as: Cell Rep. 2019 Jun 18;27(12):3486–3499.e6. doi: 10.1016/j.celrep.2019.05.079

Figure 1. Combinatorial Cellular Reprogramming with Reprogram-Seq.

Figure 1.

Analysis of single-cell transcriptomes or expert knowledge identifies a set of candidate TFs for direct reprogramming. TFs are cloned into retroviral expression vectors and induced in MEFs. In Reprogram-Seq, we performed scRNA-Seq on thousands of individual cells to identify successfully reprogrammed cells and the exogenous factors expressed in those cells. The process can then be repeated with those factors to optimize TF cocktails for direct cellular reprogramming.