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. Author manuscript; available in PMC: 2019 Jul 30.
Published in final edited form as: Cell Rep. 2019 Jun 18;27(12):3486–3499.e6. doi: 10.1016/j.celrep.2019.05.079

Figure 2. Application of Reprogram-Seq for Unbiased Reprogramming with 48 Factors.

Figure 2.

(A) Experimental design. We performed scRNA-Seq on MEFs reprogrammed with 48F, uninfected MEFs, and primary P0 mouse heart cells.

(B) The 25,776 cells were clustered and plotted using t-distributed stochastic neighbor embedding (t-SNE; MEF-derived, red; primary cells, non-red).

(C) Heatmap of cells by Myod1 expression. MEF-derived cells and Cluster 20 are indicated.

(D) Expression of Myod1 in MEF-derived cells.

(E) Differential-expression analysis of 48F in MEF-derived clusters, as compared with all other reprogrammed cells. Each dot represents a gene (colored by fold change and sized by p value).

(F) Gene ontology analysis for genes differentially expressed in Cluster 20.

(G) Heatmap of cells by Wt1 expression. Indicated is Cluster 12, which consists of MEF-derived cells and primary cells.

(H) Expression of Wt1 and Bnc1 in MEF-derived cells.