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. Author manuscript; available in PMC: 2019 Jul 30.
Published in final edited form as: Cell Rep. 2019 Jun 18;27(12):3486–3499.e6. doi: 10.1016/j.celrep.2019.05.079

Figure 4. Optimized Epicardial Reprogramming with Atf3, Gata6, and Hand2.

Figure 4.

(A) Estimates of exogenous and endogenous expression for 10F in Cluster 10 reprogrammed cells.

(B) Experimental design. We performed scRNA-Seq on uninfected MEFs and MEFs reprogrammed with 10F for 7 days, 10F for 14 days, and 3F for 7 days.

(C–F) We performed pseudotime analysis on the MEF-derived cells above and primary epicardial cells (blue). Indicated in orange are uninfected MEFs (C) and MEFs reprogrammed with 10F for 7 days (D), 10F for 14 days (E), and 3F for 7 days (F). States A–H of pseudotime are indicated in (C).

(G) Bar plot indicating the fraction of cells in each experiment that belong to the initial State A (left) or terminal State H (right) of pseudotime.

(H) Heatmap of cells by the expression of the epicardial marker gene Gpm6a (left) and Upk1b (right).