Table 7. Virulence genes present in the O26:H11 STEC MinION assemblies by in silico analysis.

Plasmid borne genes were: espP, toxB, katP, and ehxA.

Assemblies1	espI	stx2a4	stx1a5	tccP	efa1	katP
2CFSAN027343a	-	-	+	-	+	+
3CFSAN027343	-	-	+	-	+	+
2CFSAN027346a	-	-	+	+	+	+
2CFSAN027346b	-	-	+	+	+	+
3CFSAN027346	-	-	+	+	+	+
2CFSAN027350a	+	+	-	+	-	-
2CFSAN027350b	+	+	-	+	-	-
3CFSAN027350	+	+	-	+	-	-

All assemblies were positive for astA, cif, eae, ehxA (plasmid), espA, espB, espF, espJ, espP (plasmid), gad, iha, iss, lpfA, nleA, nleB, nleC, tir, and toxB (plasmid) genes.

¹Runs: a) first, and b) second run.

²1D Genomic DNA by ligation (SQK-LSK108).

³rapid sequencing kit (SQK-RAD002).

^4,5shiga toxin genes and their variants.