Table 5.
Effects of lipid peroxidation dose and BW on serum oxidative status and antibody titers of nursery pigs1
| Level of peroxidation2 | BW group3 | P-value | ||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Item | 0% | 25% | 50% | 75% | 100% | SEM | Light | Heavy | SEM | Perox4 | Linear | Quad | BW | Perox*BW |
| Vitamin E, ppm | 1.24 | 1.32 | 1.23 | 0.99 | 1.15 | 0.06 | 1.12 | 1.24 | 0.04 | 0.004 | 0.011 | 1.000 | 0.033 | 0.275 |
| 8OHdG, pg/mL5 | 1,799 | 2,034 | 1,948 | 2,002 | 1,686 | 164 | 1,987 | 1,801 | 104 | 0.530 | 0.623 | 0.120 | 0.209 | 0.636 |
| TAC, µM Trolox eq/mL6 | 100.0 | 97.1 | 89.1 | 84.7 | 92.9 | 4.13 | 88.4 | 97.1 | 2.60 | 0.069 | 0.051 | 0.102 | 0.021 | 0.842 |
| MDA, µm7 | 9.08 | 10.93 | 10.20 | 9.26 | 9.25 | 0.54 | 8.90 | 10.58 | 0.38 | 0.153 | 0.487 | 0.087 | 0.003 | 0.757 |
| Protein carbonyl, pmol/mg | 1,214 | 1,237 | 1,200 | 1,201 | 1,189 | 29.2 | 1,203 | 1,213 | 18.5 | 0.810 | 0.363 | 0.780 | 0.711 | 0.411 |
| Mhyo, S/P ratio8,9,10 | 0.164 | 0.100 | 0.12 | 0.13 | 0.17 | 0.02 | 0.13 | 0.15 | 0.01 | 0.177 | 0.600 | 0.032 | 0.265 | 0.663 |
| PCV2, S/P ratio8,9,10 | 0.28 | 0.30 | 0.31 | 0.30 | 0.33 | 0.02 | 0.32 | 0.29 | 0.01 | 0.589 | 0.177 | 0.984 | 0.111 | 0.107 |
1Each value within level of peroxidation represents a mean of 20 pigs and each value within BW group represents a mean of 50 pigs.
2Peroxidized corn oil was created by heating (65 °C) corn oil with constant air supply (20 L/min) for 12 d. Oil was stabilized with liquid antioxidant (TBHQ) after peroxidation. Treatments represent a control diet with 5% refined, bleached, and deodorized corn oil (0% peroxidation) and a diet with 5% peroxidized corn oil (100% peroxidation). Intermediate diets were created by mixing the control and peroxidized diets on-farm.
3Pigs sampled on day 33 of the study were grouped into light (mean BW was 12.0 kg) and heavy (mean BW was 21.8 kg) BW groups.
4Main effect of peroxidized corn oil. Main effects were partitioned into linear, quadratic (Quad), cubic, and fourth degree polynomials for trend analysis of peroxidation treatments
58-Hydroxy-2’-deoxyguanosine.
6Total antioxidant capacity.
7Malondialdehyde.
8Antibody titers to Mycoplasma hyopneumoniae (Mhyo) and porcine circovirus type 2 (PCV2).
9Titer results are presented as the sample to positive (S/P) ratio, calculated as the sample absorbance minus the negative control mean, divided by the difference between the positive and negative control means.
10Main effect of sampling day (P < 0.001). Titers were 0.06 vs. 0.22 for Mhyo and 0.26 vs. 0.35 for PCV2 when measured on days 33 and 43, respectively.