Table 2.
Primers used for real-time quantitative PCR
| Gene | Primer sequence (5′ to 3′) | Accession no. |
|---|---|---|
| MyHC-I | F: GTTTGCCAACTATGCTGGGG | NM_213855.1 |
| R: TGTGCAGAGCTGACACAGTC | ||
| MyHC-IIa | F: CTCTGAGTTCAGCAGCCATGA | NM214136.1 |
| R: GATGTCTTGGCATCAAAGGGC | ||
| MyHC-IIx | F: TTGACTGGGCTGCCATCAAT | NM_001104951.2 |
| R: GCCTCAATGCGCTCCTTTTC | ||
| MyHC-IIb | F: GAGGTACATCTAGTGCCCTGC | NM_001123141.1 |
| R: GCAGCCTCCCCAAAAATAGC | ||
| Myoglobin | F: GGAAGGTGGAGGCTGATGTC | NM_214236.1 |
| R: CCGTGCTTCTTCAGGTCCTC | ||
| Tnni1 | F: AGGAACACGAGGAGCGAGAG | NM_213912.3 |
| R: CACCTTGGCGTGAAGTTCC | ||
| NRF1 | F: GCCAGTGAGATGAAGAGAAACG | AK237171.1 |
| R: CTACAGCAGGGACCAAAGTTCAC | ||
| TFAM | F: GGTCCATCACAGGTAAAGCTGAA | NM_001130211 |
| R: ATAAGATCGTTTCGCCCAACTTC | ||
| COX4 | F: CCAAGTGGGACTACGACAAGAAC | AK233334.1 |
| R: CCTGCTCGTTTATTAGCACTGG | ||
| COX1 | F: ATTATCCTGACGCATACACAGCA | AJ950517.1 |
| R: ATTATCCTGACGCATACACAGCA | ||
| Cyt c | F: TAGAAAAGGGAGGCAAACACAAG | NM_001129970.1 |
| R: TAGAAAAGGGAGGCAAACACAAG | ||
| POLG | F: CTTTGAGGTTTTCCAGCAGCAG | XM_005653521.1 |
| R: GCTCCCAGTTTTGGTTGACAG | ||
| PGC-1α | F: CCCGAAACAGTAGCAGAGACAAG | NM_213963 |
| R: CTGGGGTCAGAGGAAGAGATAAAG | ||
| Sox6 | F: AAGCCACCTCTCCATTTGC | KF933861 |
| R: CCCAGTCAGCATCTTGTTGAA | ||
| MEF2C | F: CGCTCTTCATCTTGGGTCAG | NM_001044540.1 |
| R: CGTGTGTTGTGGGTATCTCG | ||
| Sp3 | F: CAGATGGTCAGCAGGTTCAG | XM_001928783.5 |
| R: TAGCAGGAGGTGTTCCAGAG | ||
| Thrap1 | F: CATCCCTGAAGCACACAGTC | XM_005669005 |
| R: AACATCGGCACCCTTGATA | ||
| TEAD1 | F: CAGGAGCAGTGCTAACAGGG | NM_001142669.1 |
| R: GGATTTTCCAAGCCGAAGCC | ||
| β-actin | F: TCCATCGTCCACCGCAAATG | XM_003357928.4 |
| R: TTCAGGAGGCTGGCATGAGG | ||
| Cyt b | F: ATGAAACATTGGAGTAGTCCTACTATTTACC | NC_000845.1 |
| R: CTACGAGGTCTGTTCCGATATAAGG | ||
| 18S rRNA | F: GGTAGTGACGAAAAATAACAATACAGGAC | NC_010448.3 |
| R: ATACGCTATTGGAGCTGGAATTACC |
MyHC = myosin heavy chain; Tnni1 = troponin 1; PGC-1α = PPAR γ coactivator 1α; Cyt b = cytochrome b; NRF1 = nuclear respiration factor 1; TFAM = mitochondrial transcription factor A; COX = cytochrome c oxidase; Cyt c = cytochrome c; POLG = DNA polymerase gamma; Sox6 = SRY-box containing gene 6; MEF2C = myocyte enhancer factor 2C; Sp3 = specificity protein 3; Thrap1 = thyroid hormone associated protein 1; TEAD1 = TEA domain transcription factor 1.