Table 3.

Primers for reverse-transcription and real-time quantitative PCR of microRNAs

MicroRNA	Primer sequences (5′ to 3′)
ssc-miR-23a	RT: GTCGTATCCAGTGCAGGGTCCGAGGTATTCGCACTGGATACGA CGGAAAT
	F: GCGATCACATTGCCAGGG
	R: AGTGCAGGGTCCGAGGTATT
ssc-miR-208b	RT: GTCGTATCCAGTGCAGGGTCCGAGGTATTCGCACTGGATACGA CACAAAC
	F: GCGCGATAAGACGAACAAAAG
	R: AGTGCAGGGTCCGAGGTATT
ssc-miR-499-5p	RT: GTCGTATCCAGTGCAGGGTCCGAGGTATTCGCACTGGATACGA CAAACAT
	F: GCGCGTTAAGACTTGCAGTG
	R: AGTGCAGGGTCCGAGGTATT
ssc-miR-133a-3p	RT: GTCGTATCCAGTGCAGGGTCCGAGGTATTCGCACTGGATACGA CCAGCTG
	F: GCGTTGGTCCCCTTCAAC
	R: AGTGCAGGGTCCGAGGTATT
U6	F: CTCGCTTCGGCAGCACA
	R: AACGCTTCACGAATTTGCGT