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. 2019 Jun 21;103(16):6505–6517. doi: 10.1007/s00253-019-09942-5

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© The Author(s) 2019

Open Access This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.

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Fig. 4 — Polyhydroxyalkanoate (PHA) yield is lower in B. thailandensis quorum sensing triple mutant compared with wild type. a, b PHA yield (g/l) and percentage PHA productivity (g/gDCB) respectively at different stages of fermentation of wild-type and triple mutant strains of B. thailandensis. c, d H′ NMR of polyhydroxybutyrate (PHB) produced by wild-type and triple mutant strains of B. thailandensis E264 respectively. PHA was extracted from lyophilised biomass by solvent extraction with chloroform followed by precipitation with ice-cold methanol. Results presented are biological triplicates (n = 3)