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. 2019 Jul 30;9:11050. doi: 10.1038/s41598-019-47491-z

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Cell viability and metabolic activity. (A) Live/dead cell staining after exposure to HA-NP suspension or leachate for 1 h. Live cells stain green and dead cells stain red (marked with a circle for better localization). Scale bar: 100 µm; (B) Metabolic activity of cells exposed to HA-NP suspension or leachate for 2, 3 and 4 h. *Significantly different from control (non-treated cells) for each time-point, (p < 0.05).