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. 2019 Jul 30;9:11028. doi: 10.1038/s41598-019-47412-0

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Expression levels of gene encoding TLR4 (relative to GAPDH). The figure reports the gene expression levels of TLR4 in normal or aneurysmatic tissue samples from 51 BAV and 25 TAV cases with AAA. The comparison of data showed significant differences among the four groups with p < 0.0001, by ANOVA test, Bonferroni corrected. Significant also were the levels of TLR4 gene expression by comparing data in normal aorta tissues from BAV and TAV cases (p = 0.0001, by t test, Welch corrected), and between two levels in aneurymatic tissue samples from TAV and BAV groups with AAA (p = 0.0001, by t test, Welch corrected). However, the lowest levels were observed in normal aorta tissues from BAV with AAA, that confirmed their systemic levels of s-TLR4. The statistical significance was detected by using t test and ANOVA test, when appropriate.