Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2019 Jul 30;9:11036. doi: 10.1038/s41598-019-46265-x

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Exon-intron architecture of the 7TM-encoding genomic region of aGPCR and its implication in aGPCR phylogeny. (A) Based on our mRNA variant analysis and publicly available genomic data the exon-intron structure of aGPCR groups is schematically presented. Alternating dark and light blue boxes represent GPS- and 7TM-encoding exons which are interrupted by introns. (B) The evolutionary history of vertebrate aGPCRs (human, mouse, chicken, zebrafish orthologs) was inferred by using the Maximum Likelihood method based on the JTT matrix-based model¹¹⁶. Thus, the 7TM domain of human, mouse, chicken, and zebrafish aGPCR orthologs were aligned and the tree with the highest log likelihood (-21466.21) is shown. Rhodopsin was used as outgroup. Initial tree(s) for the heuristic search were obtained automatically by applying Neighbor-Join and BioNJ algorithms to a matrix of pairwise distances estimated using a JTT model, and then selecting the topology with superior log likelihood value. The tree is drawn to scale, with branch lengths measured in the number of substitutions per site (next to the branches). The analysis involved 133 amino acid sequences. All positions containing gaps and missing data were eliminated. There were a total of 170 positions in the final dataset. Evolutionary analyses were conducted in MEGA7¹¹⁷.