Fig. 3.

snoRNA-guided 2′-O-methylation regulates Pxdn mRNA expression, protein levels, and peroxidase activity in vivo. a–d Hearts from WT, sno +/−, and sno −/− mice were analyzed. Plots show mean ± SEM, normalized to WT control as relative quantities (RQ). *p < 0.05 vs. WT by one-way ANOVA. a Pxdn mRNA had less Nm in both sno +/− (*p = 0.021) and sno −/− (*p = 0.0007), vs. WT. b Pxdn mRNA abundance was reduced in sno +/− (*p = < 0.001) and sno −/− (*p = 0.0019) mice, vs. WT. c PXDN protein expression was higher in sno +/− (*p = 0.0163) and sno −/− (*p = 0.0079) mice, vs. WT (by immunoblotting with normalization to total protein per lane). d Peroxidase activity was higher in sno +/− (*p = 0.0447) and sno −/− mice (*p = 0.0177) vs. WT, as measured using Amplex Red