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. 2019 Jul 30;9:11062. doi: 10.1038/s41598-019-47343-w

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Numerous alternative splicing events were detected in the time-course data for the HD-MY-Z cell line. (a) Experimental design for the grouping and subsequent analysis of the time-course microarray data with the EventPointer R package. The time points were grouped in four sets that resulted in three EventPointer comparisons. The data obtained with EventPointer was additionally filtered for p < 0.01 and absolute delta-PSI > 0.1. The selected genes were visualized at the probe set level with the GenomeGraphs package. (b) Enrichment analysis for Reactome pathways for the genes detected to be alternatively spliced in the three comparisons. The gene ratio denotes the ratio between the number of the genes associated with the term and the total number of genes associated with the term in the pathway.