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. 2019 Jul 30;10:3400. doi: 10.1038/s41467-019-11384-6

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 3 — General applicability of versatile BODIPY conjugates for multi-color SMLM. a Conventional images of BODIPY-C₁₂ green and BODIPY-C₁₂ red show co-localization of both fatty acid analogs (top). Conventional fluorescence and super-resolution images of BODIPY-C₁₂ red (bottom). b Conventional fluorescence and super-resolution images of LDs using BODIPY-NL. Zooms show super-resolution images of individual LDs that cannot be resolved with conventional fluorescence microscopy. The quantified diameters of different LDs have a mean of 125 nm. Scale bar: 1 µm, zoom: 100 nm