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. 2019 Jul 30;10:3400. doi: 10.1038/s41467-019-11384-6

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 6 — SMLM and tracking of fatty acid analogs and LysoTracker Green using D_II-states in living mammalian cells. a Conventional fluorescence image of BODIPY-C₁₂ in U2OS cells. LDs of various sizes become visible and are generally bigger compared to yeast. b Super-resolution microscopy image of D_II states of BODIPY-C₁₂. Zoom: Superposition of single-molecule localizations (red) and traces (blue) reveal the motion of fatty acid analogs along the periphery of LDs. c Conventional fluorescence image of LysoTracker Green with 488 nm excitation. d SMLM images of D_II states of LTG with 561 nm excitation. Zoom: SMLM image of an immobile lysosome. Scale bar: 5 µm; insets: 500 nm