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. 2019 Jul 30;10:3415. doi: 10.1038/s41467-019-11321-7

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 1 — Comparison of conventional and revised AAV tropism screening methods. a Following injection of AAV2/8.CMV.HI.eGFP-Cre.WPRE.SV40 (AAV8-Cre-eGFP) into C57BL/6J mice, only the subset of cells with stable, high transgene expression will show nuclear-localized eGFP. Cells with transient or low transgene expression are missed. b Injection of the same AAV8-Cre-eGFP vector into Ai14 reporter mice (containing an endogenous loxP-STOP-loxP-tdTomato locus) captures cells with stable, high transgene expression (green nuclei) as well as cells with transient or low expression of the AAV transgenes (Cre-tagged tdTomato positive cells)