Figure 5.

AATF is a central component of the protein complexes involved in SSU maturation and interacts with RNAs and proteins involved. (A–F) In our dataset, AATF interacts with the majority of the proteins known to be members of the key protein complexes involved in the maturation of ribosomal RNA^34,41. Networks were created using Cytoscape and show constituents of the tUTP (A), the UTP-B (B), the UTP-C (C), the C/D snoRNAP and MPP10 complex (D) as well as the H/A snoRNP (E) and the exosome complex (F). (stringency-eCLIP (see Suppl. Table 1): high = 665 eCLIP targets containing at least one significant peak, low = all targets identified in eCLIP; stringency AP-MS: high = log₂FC ≥2, log₁₀ p-value ≥1.3 in either AATF with or without RNAse, low = detected in our interactome with a positive FC in at least two biological replicates of AATF with or without RNAse, both compared to the respective GFP pulldown).