Table 1.
List of the primer used in this study
| Primer name and sequence | Amplicon size (bp) | Annealing temperature (°C) | References |
|---|---|---|---|
| Giardia intestinalis 18s rRNA | 350 | 67 | Rai et al. (2005) |
| F:AGCCGGACACCGCTGGCAACC | |||
| R: CGGCTGCTGGCACCAGACCTT | |||
| Giardia intestinalis tpi | 605 | 50 | Sulaiman et al. (2003) |
| External | |||
| F: AAATIATGCCTGCTCGTCG | |||
| R: CAAACCTTITCCGCAAACC | |||
| Giardia intestinalis tpi | 503 | 50 | Sulaiman et al. (2003) |
| Internal | |||
| F: CCCTTCATCGGIGGTAACTT | |||
| R: GTGGCCACCACICCCGTGCC | |||
| Giardia intestinalis gdh | 458 | 56 | Feng and Xiao (2011) |
| F:TCAACGTCAACCGCGGCTTCCGT | |||
| R: GTTGTCCTTGCACATCTCC | |||
| Giardia intestinalis Assemblage A specific | 326 | 58 | Vanni et al. (2012) |
| F: TGCTTCGGGGCGCATGCA | |||
| R: CAGGTAGCAGAGAATCCCTCG | |||
| Giardia intestinalis Assemblage B specific | 347 | 58 | Vanni et al. (2012) |
| F: ATGTGTCAGTGTGACAGTAACGT | |||
| R: GTGACTGTGCCGTTGAGGCAGT | |||
| Entamoeba histolytica | 439 | 55 | Parija and Khairnar (2007) |
| External:- | |||
| F: TAAGATGCACGAGAGCGAAA | |||
| R: GTACAAAGGGCAGGGACGTA | |||
| Internal:- | |||
| F: AAG CAT TGTTTCTAGATCTGAG | |||
| R: AAG AGG TCT AAC CGA AAT TAG | |||
| Cryptosporidium spp. | 825 | 55 | Xiao et al. (1999) |
| External:- | |||
| F:TTCTAGAGCTAATACATGCG- | |||
| R:CCCTAATCCTTCGAAACAGGA- | |||
| Internal:- | |||
| F:GAAGGGTTGTATTTATTAGATAAAG | |||
| R:AAGGAGTAAGGAACAACCTCCA | |||
| Blastocystis spp. | 462 | 55 | Yoshikawa et al. (2000) |
| F:TCTTGCTTCATCGGAGTC | |||
| R:CCTTCTCGCAGTTCTTTATC |