Table 1.
A brief summary of all methods discuss in the article
| Sl no. | Techniques | Reagents required | Procedure | Total time required | DNA yield | Specificity | Cost per sample (US $) |
|---|---|---|---|---|---|---|---|
| 1 | Chelex-100 | 5% Chelex-100 | One heating and incubation at 99 °C followed by two successive centrifugation | 30 min | 67–306 ng/µl (Sjoholm et al. 2007) | 93% (Bereczky et al. 2005; Miguel et al. 2013) | $0.16 (Strøm et al. 2014) |
| 2 | TE-Buffer | 10 mM Tris pH 8.0 with HCl, 1 mM EDTA | Incubation at 55 °C and 99 °C followed by one successive centrifugation | 45 min | 87 ng/µl (Bereczky et al. 2005) | 100% (Bereczky et al. 2005) | $1 per 96 extractions (Krystal et al. 2013) |
| 3 | Methanol | Methanol | Incubation at 97 °C without use of centrifugation | 30 min | 19.60 ng/µl (Suzanne 2016) | 73% (Bereczky et al. 2005) | – |
| 4 | Phenol–Chloroform | KCL, MgCl2, Tris–cl, Tween-20, Proteinase-K, Phenol–Chloroform, Isoamyl alcohol, 70% ethanol | Lysis for 12 h, and use of Centrifuge machine for several time | 1 day or 3 days | 87 ng/µl (Nguyen et al. 2012) | – | $5 per 96 extractions (Krystal et al. 2013) |