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. 2019 Jul 24;10:503. doi: 10.3389/fendo.2019.00503

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Copyright © 2019 Riccetti, Sperduti, Lazzaretti, Klett, De Pascali, Paradiso, Limoncella, Potì, Tagliavini, Trenti, Galano, Palmese, Satwekar, Daolio, Nicoli, Villani, Aguzzoli, Reiter, Simoni and Casarini.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Western blotting (A) and silver staining analysis (B,C) of Gonal-f® and biosimilars under non-denaturing-non reducing and denaturing-reducing conditions. Samples comprising 300 ng of each preparation, according to the quantification provided by the manufacturer, were loaded. FSH presence was detected by rabbit anti-human polyclonal primary antibody against FSHβ/FSH. Recombinant hCG was used as negative control. Samples were loaded as follows: (1) Ovaleap® batch R38915, (2) Ovaleap® batch S27266, (3) Bemfola® batch PPS30400, (4) Bemfola® batch PNS30388, (5) Bemfola® batch PNS30230, (6) Gonal-f® batch AU016646, (7) Gonal-f® batch BA045956, (8) recombinant hCG. (A) Evaluation of FSH preparations under denaturing-reducing conditions, by Western blotting, using anti-FSHβ antibody. (B) Silver staining analysis of FSH preparations under non-denaturing-non reducing conditions. (C) Analysis of FSH preparations under denaturing-reducing conditions, by silver staining.