FIG 9.

Predicted centromeres in the wheat blast B71 genome. (A) Chromosome maps showing the location of centromeres in the genome of the B71 isolate of wheat blast. The AT-rich, gene-free centromere regions are marked by a yellow star in each chromosome. (B) Synteny analysis of centromeres and their flanking regions between the Guy11 and B71 genomes showed conservation of centromere locations therein. A 200-kb region is shown with respect to the Guy11 genome assembly. (C) Scaffolds (Scaf1 to Scaf5) representing the minichromosome in B71 were analyzed and are depicted with the corresponding genes and AT richness graphs. A long AT-rich region (marked with a dark green bar) in scaffold 1 (position 40795 to position 109519) was identified and represents the putative centromere in the minichromosome in wheat blast.