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. 2019 Jul 24;10:1688. doi: 10.3389/fmicb.2019.01688

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Copyright © 2019 Woodburn, Jaynes and Clemens.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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RP557 exhibits minimal cytotoxicity. Keratinocyte and fibroblast cytotoxicity was non-invasively assayed using bioluminescent strains of human HaCaT keratinocytes (A) and murine L929 fibroblast cells (B) and viability assayed using an IVIS Lumina imaging system. Hemolysis toward human RBCs is shown in (C). Data represents the mean ±SE. Bioluminescent experiments were performed in triplicate from two independent experiments. The red blood cell viability assay involved two replicates; statistically significant in comparison to RP557 (^*P < 0.05; ^**P < 0.01, ^***P < 0.001), using one-way ANOVA followed by Tukey analysis.