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. 2019 Jul 30;9:11064. doi: 10.1038/s41598-019-47586-7

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Representative histograms of MCF-7 cells stained with Annexin V FITC and PI for the detection of cell death mechanisms for (a) control untreated group and (b) treated with free baicalin at IC50 conditions for 24 hr. (c) Shows the percentage of the apoptotic cells (both early and late apoptosis) quantified for MCF-7 and MDA-MB-231 cells after being treated for 24 hr with IC50 conditions of either free baicalin or encapsulated within F4 or F8 and compared with the control untreated group (Mean ± standard error, n = 3, Statistical significance: P value *less than 0.05, **less than 0.01, ***less than 0.001).