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. 2019 Jul 30;9:11034. doi: 10.1038/s41598-019-47513-w

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Response of the biosensor to hrMMP-8. Biosensor phase change response to increasing concentrations of hrMMP-8 (0–1000 ng/ml). Overlaid plots of raw data from a single experiment showing phase shifts recorded as a function of time after the addition of the detection antibody at t = 0 (a). Graph illustrating the relationship of relative phase change between samples containing MMP-8 and controls (Δφ) (b). Phase change measurements were made after final buffer wash (see Methods). Data are derived from 3 independent experiments each comprising parallel duplicate measurements on separate microchips and are presented as means ± SD (n = 6).