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. 2019 Jul 30;9:11034. doi: 10.1038/s41598-019-47513-w

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Analytical performance of the biosensor for the assay of salivary MMP-8 in periodontal disease. Analysis of salivary MMP-8 in healthy volunteers (N = 56), patients with gingivitis (N = 47) and patients with periodontitis (n = 65) using ELISA (a) and the biosensor assay (b). Data are means of duplicate measurements and are presented as box and whisker plots: boxes represent median (line) and interquartile ranges and whiskers the minimum and maximum range. Data was analysed using Kruskal-Wallis one-way ANOVA with Mann-Whitney U post hoc test, *P < 0.001. Correlation of salivary MMP-8 analysis of clinical samples (n = 168) using ELISA and biosensor assays (c). Data were analysed using Spearman’s Rank correlation, Rho = 0.795, P < 0.001.