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. 2019 Jul 23;28(4):1041–1049.e4. doi: 10.1016/j.celrep.2019.06.064

Figure 6.

Figure 6

LIPL-5/LIPF Functions in Coelomocytes

(A and C) Light micrographs of representative oil red O (ORO)-stained whole animals (upper panels) and densitometric quantification of staining of the two first intestinal cells after background removal (lower panels) for coelomocyte-deficient single mutants (coel.(−)) (represented twice; A and C) and coel.(−);lipl-5(ok3581) double mutants (A) or coel.(−) mutants overexpressing lipl-5 (C). Animals were fed ad libitum (AL) or subjected to bacterial deprivation (BD) for 1 day. Mean ± SD of n = 30. ns, not significant; ∗p < 0.05 by two-tailed Mann-Whitney U test. Representative of 3 biological replicates.

(B and D) Lifespan analyses of C. elegans fed AL or subjected to BD. (B) BD extended lifespan by 89% for coelomocyte-deprived animals (p < 0.0001) and by 106% for coelomocyte-deficient mutants for lipl-5(ok3581) (p < 0.0001). These two strains have similar lifespan in AL (p = 0.185), but coelomocyte-deprived mutants for lipl-5(ok3581) had lifespan increased by 12% in BD (p < 0.05) when compared with that of coelomocyte-deficient animals with a wild-type copy of lipl-5. (D) BD extended lifespan by 82% for coelomocyte-deficient animals (p < 0.0001) and by 93% for coelomocyte-deficient animals overexpressing lipl-5 driven by its own promoter (p < 0.0001). These two strains have similar lifespan in AL (p = 0.234) and in BD (p = 0.309). Mantel-Cox log-rank test (see Table S2 for number of replicates).