REAGENT or RESOURCE	SOURCE	IDENTIFIER
Bacterial and Virus Strains
Escherichia coli HT115	Ahringer library Nature 408, 325–330 (2000)	N/A
Escherichia coli RNAi lipl-1	Ahringer library Nature 408, 325–330 (2000)	N/A
Escherichia coli RNAi lipl-2	Ahringer library Nature 408, 325–330 (2000)	N/A
Escherichia coli RNAi lipl-3	Ahringer library Nature 408, 325–330 (2000)	N/A
Escherichia coli RNAi lipl-4	Ahringer library Nature 408, 325–330 (2000)	N/A
Escherichia coli RNAi lipl-5	Ahringer library Nature 408, 325–330 (2000)	N/A
Chemicals, Peptides, and Recombinant Proteins
TRI Reagent ®	Sigma-Aldrich	T9424-100ML
2X MRWB (2% formaldehyde [methanol free], 160 mM KCl; 40 mM NaCl, 4 mM EGTA, 1 mM spermidine, 0.4 mM spermine, 30 mM PIPES, pH 7.4, and 0.2% β-mercaptoethanol)	O’Rourke et al., 2009	N/A
Critical Commercial Assays
Gibson Assembly Kit	NEB	E5510S
RNeasy Mini Kits	QIAGEN	74104
iScript cDNA Synthesis kit	Bio-Rad	1708890
Experimental Models: Organisms/Strains
N2 Bristol	CGC	N2
glp-1(e2141ts)III	Gift from Kenyon Lab	CF1903
daf-2(e1370)III	CGC	CB1370
lipl-5(ok3581)V	CGC	RB2573
lynEx03 = [(pAB02(lipl-5p::lipl-5::td-tomato) and co-injection marker myo-2p::mCherry]	UMS3421	MCP39
lipl-5(ok3581)V;lynEx03	Our lab	HGA2912
unc-119(ed3)III;pwIs50	CGC	RT258
pwIs50 = [lmp-1::GFP + Cbr-unc-119(+)]
lipl-5::td-tomato	UMS3421	MCP34
lipl-5::td-tomato;pwIs50	Our lab	HGA2914
coel.(-)	Gift from Fares Lab	NP717
coel.(-);lipl-5(ok3581)V	Our lab	HGA2915
coel.(-);lynEx03	Our lab	HGA2916
lipl-5(ok3581)V;lynEx04	Our lab	HGA2917
lynEx04 = [(pAB02(pcc1::lipl-5::td-tomato) and co-injection marker myo-2p::mCherry]
lipl-5(bab-8)V	UMS3421	MCP12
lipl-5(ok3581)V;lynEx02	Our lab	HGA8021
lynEx02 = [(pAB01(ges-1p::lipl-5::gfp) and co-injection marker myo-2p::mCherry]
Oligonucleotides
See Table S3 for oligonucleotide information		N/A
Recombinant DNA
pHD43 plasmid	kindly provided by Hanna Fares, University of Arizona Cancer Center	N/A
pPD95.75-Pges-1 plasmid	kindly provided by Hideki Inoue, Pittsburgh University	N/A
pCFJ90: myo-2p::mCherry	Addgene	http://www.addgene.org/19327/
Software and Algorithms
ImageJ		https://imagej.nih.gov/ij/
two-tailed Mann-Whitney U test	This paper	https://www.socscistatistics.com/tests/mannwhitney/default2.aspx
two-tailed Student’s t test	This paper	https://www.xlstat.com/fr/
Mantel–Cox log-rank	This paper	https://www.xlstat.com/fr/
WinCATS software	This paper	https://www.camag.com/en/tlc_hptlc/supportservices/software/wincats_releases.cfm?command=archive