Figure 5.

FL-Mediated Expansion of Intratumoral CD103⁺ DCs Inhibits Tumor Growth and Augments Infiltration of CD8⁺ TILs to Peritoneal Cavities of ID8-T Tumor-Bearing Mice

(A and B) Evaluation of tumor-infiltrating CD8⁺Ly6C⁺ T cells in peritoneal washes after OVV treatment alone or in combination with the FL cytokine (n = 5 mice/group). Representative flow cytometry staining (A) and relative proportions of CD8⁺Ly6C⁺ TILs (B) are shown. (C) Survival of ID8-T tumor-bearing C57BL/6 mice (n = 5–10 mice/group) after OVV and OVV plus FL treatment combinations. Survival was defined as the point where mice were killed because of extensive tumor burden. Kaplan-Meier survival plots were prepared, and significance was determined using the log rank method. **p < 0.01, ***p < 0.001. (D and E) Representative flow cytometry staining (D) and relative proportions of a tumor-infiltrating B8R vaccinia virus-specific tetramer⁺ subset of CD8⁺ TILs (E). (F and G) Representative flow cytometry staining (F) and relative proportions of WT1 tetramer⁺CD8⁺ TILs (G). (H and I) Evaluation of tumor-infiltrating CD8⁺Ly6C⁺ T cells in peritoneal washes after OVV-CXCR4-A treatment alone or in combination with the FL cytokine (n = 3–5 mice/group). Representative flow cytometry staining (H) and relative proportions of the CD8⁺Ly6C⁺ TILs (I) regimen are shown. (J) Survival of ID8-T tumor-bearing C57BL/6 mice (n = 5–10 mice/group) after OVV-CXCR4-A and OVV-CXCR4-A plus FL treatment combination. Kaplan-Meier survival plots were prepared, and significance was determined using the log rank method. **p < 0.01, ***p < 0.001. (K and L) Representative flow cytometry staining (K) and relative proportions of a tumor-infiltrating B8R vaccinia virus-specific tetramer⁺ subset (L) after combined treatment with OVV-CXCR4-A and FL. (M and N) Representative flow cytometry staining (M) and relative proportions of WT1 tetramer⁺CD8⁺ TILs (N).