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. 2019 Apr 23;220(5):882–891. doi: 10.1093/infdis/jiz195

Figure 6.

Figure 6.

Acquisition of the region of difference 2 (RD2) element alters the group A Streptococcus (GAS) transcriptome. A, Results of RNA sequencing analysis comparing the parental M28 strain MGAS6180 and its RD2-deletion mutant derivative (M28ΔRD2) during exponential phase growth in Todd-Hewitt broth containing 0.2% yeast extract. Each circle represents messenger RNA (mRNA) from a single gene. Genes are arranged in the same order as they appear in the chromosome. Select virulence factor–encoding mRNAs are colored and labeled. Dashed lines indicate ±2-fold. B, Findings of TaqMan-based quantitative reverse transcription polymerase chain reaction analysis. The abundance of endoS mRNA was determined from triplicate exponential phase GAS cultures of each indicated strain, run in duplicate. Shown is the mean (± standard deviation). P < .001 (via overall analysis of variance). Pair-wise strain comparisons with the parental isolate were investigated using the Tukey multiple comparisons test. *P < .05.